Skip to Main content Skip to Navigation
New interface
Journal articles

Oxidative stress response in pulmonary cells exposed to different fractions of PM2.5-0.3 from urban, traffic and industrial sites

Abstract : The aim of this work was to study the relationship between oxidative stress damages and particulate matter (PM) chemical composition, sources, and PM fractions. PM2.5-0.3 (PM with equivalent aerodynamic diameter between 2.5 and 0.3 μm) were collected at urban, road traffic and industrial sites in the North of France, and were characterized for major and minor chemical species. Four different fractions (whole PM2.5-0.3, organic, water-soluble and non-extractable matter) were considered for each of the PM2.5-0.3 samples from the three sites. After exposure of BEAS-2B cells to the four different fractions, oxidative stress was studied in cells by quantifying reactive oxygen species (ROS) accumulation, oxidative damage to proteins (carbonylated proteins), membrane alteration (8-isoprostane) and DNA damages (8-OHdG). Whole PM2.5-0.3 was capable of inducing ROS overproduction and caused damage to proteins at higher levels than other fractions. Stronger cell membrane and DNA damages were found associated with PM and organic fractions from the urban site. ROS overproduction was correlated with level of expression of carbonylated proteins, DNA damages and membrane alteration markers. The PM2.5-0.3 collected under industrial influence appears to be the less linked to cell damages and ROS production in comparison with the other influences.
Document type :
Journal articles
Complete list of metadata

https://hal-ulco.archives-ouvertes.fr/hal-03821902
Contributor : Frédéric Ledoux Connect in order to contact the contributor
Submitted on : Thursday, October 20, 2022 - 8:22:52 AM
Last modification on : Tuesday, October 25, 2022 - 8:32:39 AM

Files

 Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed until : 2023-04-20

Please log in to resquest access to the document

Identifiers

Collections

Citation

Lamia Moufarrej, Anthony Verdin, Fabrice Cazier, Frédéric Ledoux, Dominique Courcot. Oxidative stress response in pulmonary cells exposed to different fractions of PM2.5-0.3 from urban, traffic and industrial sites. Environmental Research, 2023, 216 (Part 2), pp.114572. ⟨10.1016/j.envres.2022.114572⟩. ⟨hal-03821902⟩

Share

Metrics

Record views

0