Skip to Main content Skip to Navigation
Journal articles

Differentiating between fresh and frozen-thawed fish fillets by mitochondrial permeability measurement

Abstract : In this study, we investigated the properties of mitochondria to discriminate fresh from frozen-thawed fish fillets. Mitochondria were isolated from gilthead seabream fillets and the impact of freezing was evaluated by measuring the permeability of mitochondrial membranes. Freezing led to permeabilization of mitochondrial inner membranes to reduced nicotinamide adenine dinucleotide (NADH). The increase in permeability related to freezing shock was compared to the physiological permeabilization of mitochondria isolated from gilthead seabream fillets stored at 4°C. Two approaches were chosen to measure the increase in permeability: a spectrophotometric method to measure the consumption of NADH by complex I, and an oxygraphic method to measure O2 consumption by respiratory chains after exposure of mitochondria to NADH. Mitochondria isolated from frozen-thawed fillets were highly permeable to NADH and were no longer sensitive to a membrane permeabilizing agent: alamethicin. Altogether, our scientific approach allowed us to discriminate mitochondria isolated from fillets that have been exposed or not to a freezing shock (-80°C) and thus to discriminate between fresh and frozen-thawed fish fillets.
Document type :
Journal articles
Complete list of metadata

https://hal-ulco.archives-ouvertes.fr/hal-03699126
Contributor : Thierry GRARD Connect in order to contact the contributor
Submitted on : Monday, June 20, 2022 - 9:11:39 AM
Last modification on : Wednesday, June 22, 2022 - 2:37:29 PM

File

 Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed until : 2022-12-19

Please log in to resquest access to the document

Identifiers

Citation

Tiffanie Bouchendhomme, Méline Soret, Anne Devin, Philippe Pasdois, Thierry Grard, et al.. Differentiating between fresh and frozen-thawed fish fillets by mitochondrial permeability measurement. Food Control, Elsevier, In press, ⟨10.1016/j.foodcont.2022.109197⟩. ⟨hal-03699126⟩

Share

Metrics

Record views

0